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Real Time
PCR

Real-Time PCR

Highly efficient Applied Biosystems real-time PCR solutions minimize complexities to help maximize your time and effort

Real-time PCR, also known as quantitative PCR (qPCR), is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets. We developed powerful assay design algorithms, optimized master mixes, created intuitive data analysis software, and built smart instrumentation to help harness the power of real-time PCR across a rich and diverse set of applications. However, our solutions go a step further: every product has been designed with efficiency baked in. And together they work synergistically to bring you a simpler, more flexible, and highly efficient real-time PCR workflow

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Elements of the Applied Biosystems real-time PCR ecosystem

TaqMan Real-time PCR assays

Industry-leading choice for 5ʹ nuclease real-time PCR assays. >20 million predesigned assays (primer/probe sets) available in five formats and unlimited content configurations. Custom designs and individual primers/probes also available.


QuantStudio Real-time PCR systems

With more than 25 years of qPCR innovation, Applied Biosystems continues to lead the way in performance, reliability, and world-class support. From low- to high-throughput solutions, there’s a QuantStudio real-time PCR system that’s just right for your research.


Real-time PCR reagents & kits

TaqMan master mixes are optimized to work with our TaqMan Assays to provide optimal sensitivity, specificity, and dynamic range. Our SYBR portfolio offers a variety of features providing a wide range of instrument compatibility.


Real-time PCR data analysiss

From gene expression to Ct analysis and copy number variation determination, we have developed robust analysis software for nearly every real-time PCR application. Plus, all our analysis tools have been optimized for use with our TaqMan Assays on Applied Biosystems Real-time PCR instruments.


Featured real-time PCR solutions

PowerTrack SYBR Green Master Mix

Two-color tracking dye system indicates where pipetting has occurred, reducing potential errors.

 
QuantStudio 6 & 7 Pro systems

For research of genetics, environment, lifestyle factors and beyond

 
 
Pre-spotted 384-well TaqMan Array cards

Can you imagine loading a 384-well array in just a few minutes? We did. Then we made it happen. Discover a simplified real-time PCR benchtop workflow with TaqMan Array cards.

 

Real-time PCR applications

Gene expression (mRNA) analysis

Gene expression is the most common application for real-time PCR due to its efficiency at detecting and quantifying target genes, even at very low expression levels.

 
Genetic variation analysis

Forensic DNA analysis and phenotyping, from genotyping to STRs to mitochondrial DNA

 
MicroRNA (miRNA) analysis

miRNAs are small, noncoding RNA molecules that direct post-transcriptional suppression of gene expression. Our solutions enable miRNA research from isolation through discovery, profiling, quantitation, verification, and functional analysis.

 
Mutation detection

Real-time PCR is a widely used technology for mutation detection that not only provides excellent sensitivity and specificity when paired with our gold-standard TaqMan Assays, but also provides rapid results and is easy to perform when compared to other methods. Explore these solutions:

 
Protein expression analysis

TaqMan Protein assays combine the sensitive, specific protein-binding capabilities of antibodies with the superior relative quantitation capabilities of 5' nuclease real-time PCR. They enable fast, easy identification and relative quantification of proteins from small sample sizes or limited numbers of cells—all in a homogeneous assay format.

 
Take a virtual demo of our Quantstudio System Request Demo
 
Explore QPCR Solutions for our SARS-COv-2 Research
 
QPCR Handbook 2.0 Digital Experience
 
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Every client has a unique requirement. Working together we’ll discover the right solution which fits in your budget to reach the desired goal.

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